UCE target enrichment of Donquickeia_sp.2_CNIN4133: whole organism - SRA

SRX11210916: UCE target enrichment of Donquickeia_sp.2_CNIN4133: whole organism
1 ILLUMINA (Illumina NovaSeq 6000) run: 869,466 spots, 243.3M bases, 91Mb downloads

Design: DNA was extracted from voucher specimens following a nondestructive technique using the EZ-10 Spin Mini preps Kit (Bio Basic, Toronto, Canada). We followed Branstetter et al.s (2017) protocol for library preparation and capture and enrichment of UCE loci. All DNA extractions were quantified using Qubit 2.0 (Life Technologies). For each sample, up to 50 ng of extracted DNA was fragmented to an average fragment distribution of 400600 bp using either a Bioruptor Pico sonication device (Diagenode Inc., Danville, NJ, USA) or a Qsonica Q800R sonicator (Qsonica LLC, Newton, CT, USA). Genomic libraries were constructed using the Kapa Hyper Prep library preparation kit (Kapa Biosystems Inc., Wilmington, MA, USA) and TruSeq-style dual-indexing barcodes (Glenn et al., 2019). Target enrichment of the DNA libraries was performed using the two enhanced commercial myBaits UCE sets (1.5Kv1 and 2.5Kv2; ArborBiosciences, Ann Arbor, MI, USA) that were specifically designed for the order Hymenoptera and developed by Faircloth et al. (2015) and Branstetter et al. (2017). Pooled enriched libraries were sent to two different locations for sequencing services. Sequencing was performed for a first pool with an Illumina NextSeq v2 300 cycle kit (150-bp pairedend, Illumina Inc., San Diego, CA, USA), whereas the remaining enriched libraries were sequenced using an Illumina NovaSeq 6000 (150-bp paired-end, Illumina Inc., San Diego, CA, USA).

Submitted by: Instituto de Biologia, UNAM

Study: Ultraconserved elements-based systematics reveals evolutionary patterns of host-plant family shifts and phytophagy within a predominantly parasitoid wasp family

PRJNA733199 • SRP325387 • All experiments • All runs

show Abstracthide Abstract

We conducted a comprehensive phylogenomic study for the doryctine gall-associated genera, with an emphasis on Allorhogas, using ultraconserved elements (UCEs). Based on this estimate of phylogeny we evaluated their taxonomic composition, estimated the timing of origin of the gall-associated clade and divergence of its main subclades, and performed ancestral state reconstruction analyses for life history traits related to their host-plant association.

Sample:

SAMN19775227 • SRS9265224 • All experiments • All runs

Organism: Donquickeia sp. 2 ESS-2021

Library:

Name: Donquickeia_sp.2_CNIN4133

Instrument: Illumina NovaSeq 6000

Strategy: WGS

Source: GENOMIC

Selection: Hybrid Selection

Layout: PAIRED

Runs: 1 run, 869,466 spots, 243.3M bases, 91Mb

Run	# of Spots	# of Bases	Size	Published
SRR14895575	869,466	243.3M	91Mb	2021-06-24

ID:: 14953060

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity